Transgenic trait deployment using designed nucleases
نویسندگان
چکیده
منابع مشابه
Enhancing gene targeting with designed zinc finger nucleases.
Gene targeting—the process of gene replacement by homologous recombination—is a very useful but typically inefficient technique (1). A general method for improving the efficiency of gene targeting would be valuable in many circumstances, as would the extension of this genetic tool to a broader range of organisms. Model experiments have demonstrated that a double-strand break (DSB) in the chromo...
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Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate targeted-gene inactivation for an endogenous gene in Arabidopsis using zinc finger nucleases (ZFNs). Engineered ZFNs for a stress-response regulator, the ABA-INSENSITIVE4 (ABI4) gene, cleaved their recognition sequences specifically in vitro, and Z...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2015
ISSN: 1467-7644
DOI: 10.1111/pbi.12457